Fig. 5. EZH2 inhibition did not affect chondrosarcoma survival. (A) Chondrosarcomas were cultured with EPZ6438 or GSK126 for one week. At the end of treatments, H3K27me3 was determined by Western-blot. (B-C) Cells were cultured with DZNep, EPZ6438 or GSK126. At the end of treatments (one week), survival fraction and percentage of apoptotic cells were evaluated by counting adherent cells (B) and by Apo2.7 staining (C), respectively. Data are expressed as means + SEM (n=3). *: p-value < 0.05 (D-E) Cells were transfected with siRNA-control or siRNA-EZH2 and incubated 72h. Then, EZH2 expression was determined at protein level by western-blot (D). Cell survival was assayed by ATP measure using Cell Titer Glo Assay (E).